MultiVis Introduction

SPRITE (Split-Pool Recognition of Interactions by Tag Extension) is a technique used to map the three-dimensional organization of the genome by capturing and sequencing DNA fragments that are physically proximal within the nucleus. The SPRITE pipeline typically utilizes R to generate static heatmaps of these interactions; however, these heatmaps are not interactive, provide no real-time ability to change downweight parameters, and do not allow on-the-fly modifications to the output process.

The image above illustrates the workflow of MultiVis, a specialized visualization framework designed to address limitations in traditional SPRITE-based analyses. Typically, SPRITE, RD-SPRITE, or scSPRITE experiments generate a cluster file that captures genomic interaction information. This cluster file can be provided as an input to our Generate MultiVis Directory tool. Running this tool produces a fully configured MultiVis directory, which you can then load into MultiVis for further exploration.

Unlike the standard SPRITE pipeline, MultiVis allows you to dynamically modify key analysis parameters, such as downweighting methods, output processes, and resolution settings, directly through its user-friendly interface. This interactive approach eliminates the need for repeatedly running command-line scripts, enabling real-time adjustments and streamlined data exploration. By providing instant feedback on parameter changes, MultiVis makes it easier to investigate and refine the three-dimensional organization of the genome captured by SPRITE-based experiments.